Abstract
The purpose of this study was to evaluate the probiotic characteristics and neuroprotective effects of bacteria isolated from Korean fermented foods. Three bacterial strains (Lactobacillus fermentum KU200060, Lactobacillus delbrueckii KU200171, and Lactobacillus buchneri KU200793) showed potential probiotic properties, such as high tolerance against artificial gastric juice and bile salts, sensitivity to antibiotics, nonproduction of carcinogenic enzymes, and high adhesion to intestinal cells. Heat-killed L. fermentum KU200060 and L. buchneri KU200793 showed higher antioxidant activity than heat-killed L. delbrueckii KU200171. The conditioned medium (CM) was used to evaluate the reaction between HT-29 cells and each heat-killed strain. All CMs protected SH-SY5Y cells from 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity. The expression of brain-derived neurotropic factor (BDNF) mRNA in HT-29 cells treated with CM containing heat-killed L. buchneri KU200793 was the highest. The CM significantly reduced the Bax/Bcl-2 ratio and increased BDNF mRNA expression in SH-SY5Y cells treated with MPP+. These results indicate that L. buchneri KU200793 can be used as a prophylactic functional food, having probiotic potential and neuroprotective effects.
Highlights
Probiotics are defined as living microorganisms administered in appropriate amounts that confer a beneficial effect on the host [1]
The purpose of this study was to identify L. fermentum KU200060, L. delbrueckii KU200171, and L. buchneri KU200793 isolated from kimchi and to evaluate its probiotic potential, including its tolerance to gastric acid and bile salt conditions, enzyme production, adhesion ability to intestine, and antibiotic susceptibility characteristics
L. paraplantarum SC61 and Pediococcus pentosaceus SC28 have higher adhesion abilities to HT-29 cell (6.26% and 4.03%) than L. rhamnosus GG (2.74%) [27]. These results indicate that L. buchneri KU200793 had higher adhesion ability to HT-29 cell than other probiotic strains and could attach and colonize the human intestinal epithelial cells
Summary
Probiotics are defined as living microorganisms administered in appropriate amounts that confer a beneficial effect on the host [1]. To be used as probiotics, microorganisms should have the capacity to withstand physical and chemical conditions in the human body and to colonize and adhere to the intestinal epithelial cells [2]. The conceptual framework of the gut-brain-axis (GBA) has existed for decades [8]. Bidirectional communication between gut microbiota and the components of the GBA influences normal homeostasis and may contribute to neurodegenerative diseases [9]. A recent study showed that gut-brain communication aids in the modulation of immune activity. Chronic proinflammatory immune activity is increasingly recognized as the central element of neurodegenerative disorders, such as Parkinson’s disease (PD) inflammation in the intestine appears related to their pathogenesis [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
