Abstract
Lactic acid bacteria (LAB) are one of group probiotic bacteria, which have a positive impact on human and animal health. The aim of this research was to determine the potency lactic acid bacterial isolate from LABi cattle gastric as a probiotic that have resistance to low pH and sodium deoxycholate. The study was started with the cultivation test that aims to made sure uncontaminated isolates of microorganisms, including test for growth response in MRS broth medium, test on catalase production, and Gram stain. Its potency for probiotic development was tested by growing of isolate in MRS broth medium with low pH conditions (pH 2, 3, 4) and in MRS medium supplemented with various concentration of sodium deoxycholate (0,2 mM, 0,4 mM, 0,6 mM). The result showed that isolate from LABi cattle gastric was able to grow well in MRS broth medium under anaerobic condition. In addition, it also showed catalase negative and Gram-positive bacteria. In the main experiment, this isolate was found to be resistant to low pH conditions (up to pH 2) and high concentration of sodium deoxycholate (up to 0,6 mM). These results indicate that the isolate the lactic acid bacteria have resistance to low pH and the sodium deoxycholate, which is the main requirement of the probiotic bacteria
Highlights
Lactic acid bacteria (LAB) is a bacterium that is safe for its host and is able to produce compounds that can inhibit the growth of pathogenic bacteria [1]
The results of the test of catalase on the 5 isolates of lactic acid bacteria when ditetetsi with the 10% H2O2 does not produce bubbles of oxygen gas (O2), this shows that the 5 isolates of lactic acid bacteria react negatively to a test of catalase
Lactic acid bacteria are generally classified into a group of anaerobic bacteria, these bacteria do not have the enzyme catalase which can break down H2O2 into H2O and O2
Summary
Lactic acid bacteria (LAB) is a bacterium that is safe for its host and is able to produce compounds that can inhibit the growth of pathogenic bacteria [1]. In general the LAB is defined as a group of Grampositive bacteria, it does not produce spores, spherical or rod, catalase negative, have no cytochrome, aerotolerant anaerobic, microaerophilic bacteria until, and requires the nutrients the complex such as amino acids, vitamins (B1, B6, B12, and biotin), purin, pirimidin[2];[3]. Generally the species LAB more tolerant of environmental conditions pH acidic [5]. This relates to the conditions of the digestive tract of animals that belong to have the condition with low pH or acid. A cow or a healthy ruminant, normally have a different pH along thedigestion channel, starting from reticulum-gastrict pH 5.3-6.5; abomasum pH
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