Probiotic potential of Lactobacillus paraplantarum BT‐11 isolated from raw buffalo ( Bubalus bubalis ) milk and characterization of bacteriocin‐like inhibitory substance produced

Abstract

This study aimed to isolate bacteriocin-like inhibitory substance (BLIS) producing strains from raw buffalo milk and characterize their probiotic and antimicrobial potential. The BT-11 isolate was identified through 16S rDNA gene sequencing and recA gene amplification as Lactobacillus paraplantarum. The BT-11 strain survived in low pH (2, 3) and bile (0.5, 1, and 2%) for 3 hr of incubation. The strain exhibited adhesion to Caco-2 cells (69.85%), auto-aggregation (89.74%), and hydrophobicity (81.76%). The BLIS produced by BT-11 showed antibacterial activity against indicator bacteria including multidrug-resistant pathogens. Time–kill kinetic analysis revealed the reduction in the growth of Staphylococcus aureus and Salmonella typhimurium (>3 Log CFU/ml) in time-dependent manner. BLIS showed biopreservative effect in meat contaminated with Listeria monocytogenes. The results concluded that L. paraplantarum BT-11 is potential probiotic strain with potential to produce BLISs. Practical applications Raw buffalo milk represents excellent source of unexplored potential probiotics with antimicrobial characteristics. In this study, the Lactobacillus paraplantarum BT-11 was isolated from raw buffalo milk and its probiotic potential and bacteriocin-like inhibitory substance (BLIS) was characterized. The results demonstrated that L. paraplantarum BT-11 is promising potential probiotic. The BLIS produced by L. paraplantarum BT-11 showed antagonistic activity against pathogens including multidrug-resistant foodborne bacteria. BLIS also showed biopreservative potential in meat against Listeria monocytogenes. L. paraplantarum and its antimicrobial compound can be used as bio preservative agent in food safety applications.