Abstract
The purpose of this study was to establish the probiotic potential of lactic acid bacteria (LAB) starter cultures, Lb. plantarum MNC 21, L. lactis MNC 24, and W. confusa MNC 20, isolated from a traditionally fermented sorghum-millet beverage from Uganda. The cultures were examined for tolerance to acid and bile salts, bile salt hydrolase (BSH) activity, antibiotic susceptibility, biogenic amine production, mucin degradation, hydrophobicity, auto-aggregation, adherence to the ileum, coaggregation, and antimicrobial properties against selected pathogenic species. Lb. rhamnosus yoba 2012, a known probiotic, was the reference. The isolates were tolerant to acid (pH = 3) and bile (1%). W. confusa MNC 20 and Lb. plantarum MNC 21 exhibited medium BSH activity (11–15 mm diameter of hydrolysis zone) while L. lactis and Lb. rhamnosus yoba 2012 exhibited low BSH activity (<10 mm diameter of hydrolysis zone). All isolates lacked mucolytic activity. Lb. plantarum MNC 21 and W. confusa MNC 20 produced agmatine. The candidate and reference microorganisms were resistant to 10 of 21 and 5 of 21 antibiotics, respectively. The isolates exhibited hydrophobic, auto-aggregation and coaggregation properties. These three properties were exhibited more (p < 0.05) by the reference than the potential probiotics. The ability of the potential probiotics to attach onto the goat ileum (7.3–8.0 log cfu/cm2) was comparable to that of Lb. rhamnosus yoba 2012 (7.6 log cfu/cm2). The four LAB inhibited E. coli, S. aureus, and S. enterica to the same extent (p < 0.05). The findings indicated potential probiotic activity of the starter cultures. However, further in vivo examination of these isolates is required to confirm their probiotic capabilities.
Highlights
E purpose of this study was to establish the probiotic potential of lactic acid bacteria (LAB) starter cultures, Lb. plantarum MNC 21, L. lactis MNC 24, and W. confusa MNC 20, isolated from a traditionally fermented sorghum-millet beverage from Uganda. e cultures were examined for tolerance to acid and bile salts, bile salt hydrolase (BSH) activity, antibiotic susceptibility, biogenic amine production, mucin degradation, hydrophobicity, auto-aggregation, adherence to the ileum, coaggregation, and antimicrobial properties against selected pathogenic species
Lb. plantarum MNC 21 and W. confusa MNC 20 produced agmatine. e candidate and reference microorganisms were resistant to 10 of 21 and 5 of 21 antibiotics, respectively. e isolates exhibited hydrophobic, auto-aggregation and coaggregation properties. ese three properties were exhibited more (p < 0.05) by the reference than the potential probiotics. e ability of the potential probiotics to attach onto the goat ileum (7.3–8.0 log cfu/ cm2) was comparable to that of Lb. rhamnosus yoba 2012 (7.6 log cfu/cm2). e four LAB inhibited E. coli, S. aureus, and S. enterica to the same extent (p < 0.05). e findings indicated potential probiotic activity of the starter cultures
LAB are known to contribute to the flavor profile and safety of fermented foods as well as International Journal of Microbiology promote health by acting as probiotics [24, 25]. ree LAB isolates from Obushera, Lactobacillus (Lb.) plantarum MNC 21, Lactococcus (L.) lactis MNC 24, and Weissella (W.) confusa MNC 20, possess excellent starter culture properties for the beverage [23, 26]. ese cultures have been piloted for the commercial production of Obushera and have promise for use in related fermented food products
Summary
Lb. plantarum MNC 21, L. lactis MNC 24, and W. confusa MNC 20 were isolated from Obushera [23]. Stock cultures were stored at −80°C in Ringer’s solution containing 15% glycerol. From the stock cultures of Lb. plantarum MNC 21, L. lactis MNC 24, W. confusa MNC 20, and Lb. rhamnosus yoba 2012, 0.1 mL was delivered into 100 mL of sterile MRS broth (Laboratorios CONDA, Madrid, Spain) and incubated at 30°C for 24 h. For the E. coli ATCC 25922, S. aureus ATCC 25923, and S. enterica, 0.1 mL of stock cultures was separately inoculated in 100 mL of sterile brain heart infusion (BHI) broth (Laboratorios CONDA, Madrid, Spain) and incubated at 30°C for 24 h. E cell pellets were suspended in 100 mL of sterile Ringer’s solution (Oxoid Ltd, Basingstoke, Hampshire, England) and used for the different screening assays. From the fresh culture suspensions, 1 mL of each isolate was separately added to
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