Abstract
Salmonella enterica serovar 4,[5],12:i:- (S. 4,[5],12:i:-) is an emerging foodborne pathogen causing salmonellosis in humans and animals. Probiotic Lactobacillus rhamnosus GG (LGG) is an effective strategy for controlling enteric infections through maintaining gut microbiota homeostasis and regulating the intestinal innate immune response. Here, LGG was orally administrated to newly weaned piglets for 1 week before S. 4,[5],12:i:- challenge. S. 4,[5],12:i:- challenge led to disturbed gut microbiota, characterized by increased levels of Psychrobacter, Chryseobacterium indoltheticum, and uncultured Corynebacteriaceae populations, as well as an aberrant correlation network in Prevotellaceae NK3B31 group-centric species. The beneficial effect of LGG correlated with attenuating the expansion of Prevotellaceae NK3B31 group. Fusobacterium only found in the pigs treated with LGG was positively correlated with Lactobacillus animalis and Propionibacterium. Administration of LGG induced the expansion of CD3-CD19-T-bet+IFNγ+ and CD3-CD19-T-bet+IFNγ- cell subsets in the peripheral blood at 24 h after a challenge of S. 4,[5],12:i:-. S. 4,[5],12:i:- infection increased the population of intraepithelial CD3-CD19-T-bet+IFNγ+ and CD3-CD19-T-bet+IFNγ- cells in the ileum; however, this increase was attenuated via LGG administration. Correlation analysis revealed that LGG enriched Flavobacterium frigidarium and Facklamia populations, which were negatively correlated with intraepithelial CD3-CD19-T-bet+IFNγ+ and CD3-CD19-T-bet+IFNγ- cells in the ileum. The present data suggest that probiotic LGG alters gut microbiota with associated CD3-CD19-T-bet+IFNγ+/- cell subset homeostasis in pigs challenged with S. enterica 4,[5],12:i:-. LGG may be used in potential gut microbiota-targeted therapy regimens to regulate the specific immune cell function and, consequently, control enteric infections.
Highlights
The non-typhoidal Salmonella (NTS) disease imposes a substantial burden of pediatric and adult morbidity and mortality globally and is estimated to cause 93.8 million human infections and 155,000 deaths annually worldwide (Majowicz et al, 2010; Gilchrist et al, 2015)
We previously reported that Lactobacillus rhamnosus GG (LGG) consumption regulates T-bet expression in the ileum of pigs, ameliorating intestinal inflammation caused by S. enterica serovar Infantis (Yang et al, 2017)
Intraepithelial ILC1s and IFNγ-producing ILC3s accumulated in mice, and blocking IFNγ ameliorated intestinal inflammation in a mouse model of colitis (Buonocore et al, 2010). These results indicate that LGG administration attenuates the increase in the proportion of intraepithelial CD3−CD19−T-bet+IFNγ+ cells and CD3−CD19−T-bet+IFNγ− cells owing to S. 4,[5],12:i:in the ileum, potentially aiding the amelioration of chronic intestinal inflammation
Summary
The non-typhoidal Salmonella (NTS) disease imposes a substantial burden of pediatric and adult morbidity and mortality globally and is estimated to cause 93.8 million human infections and 155,000 deaths annually worldwide (Majowicz et al, 2010; Gilchrist et al, 2015). 4,[5],12:i:-) is an emerging foodborne pathogen, causing salmonellosis in domestic and wild animals as well as acute gastroenteritis in humans (Gallati et al, 2013; Mascaro et al, 2017). S. 4,[5],12:i:- is believed to be a monophasic variant of S. enterica subsp. Typhimurium) and has lost the genetic basis for encoding the phase 2 flagellin (Ido et al, 2014). 4,[5],12:i:isolates causing foodborne salmonellosis outbreaks in humans often turned out to be pigs and pork products (Rodriguez et al, 2012). S. 4,[5],12:i:- risk to consumers via pork, poses a considerable threat to public health
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
