Abstract
Probiotics are well known for their beneficial effects for animals, including humans and livestock. Here, we tested the probiotic activity of Lactobacillus paracasei expressing 3D8 scFv, a nucleic acid-hydrolyzing mini-antibody, in mice intestine. A total of 18 fecal samples derived from three different conditions at two different time points were subjected to high-throughput 16S ribosomal RNA (rRNA) metagenomic analyses. Bioinformatic analyses identified an average of 290 operational taxonomic units. After administration of L. paracasei, populations of the probiotics L. paracasei, Lactobacillus reuteri, and Pediococcus acidilactici increased, whereas the population of harmful bacteria such as Helicobacter species decreased. Furthermore, continuous administration of L. paracasei resulted in L. paracasei emerging as the dominant probiotic after competition with other existing probiotics. Expression of 3D8 scFv protein specifically increased the population of P. acidilactici, which is another probiotic. In summary, our results showed that L. paracasei expressing 3D8 scFv protein enhanced probiotic activity in mice intestine with no observable side effects. Thus, the system developed in this study may be a good tool for the expression of recombinant protein using probiotics.
Highlights
The microbiota in the gastrointestinal (GI) tracts of mammals are complex, and their composition can be influenced by several factors, including diets and environmental changes
Our results showed that L. paracasei expressing 3D8 scFv promoted probiotic activity in the mouse intestine
Samples were from mice fed L. paracasei, and TG samples were from mice fed L.paracasei expressing
Summary
The microbiota in the gastrointestinal (GI) tracts of mammals are complex, and their composition can be influenced by several factors, including diets and environmental changes. Among known microbiota in the GI tracts, some bacteria species are regarded as probiotics, which can be defined as microorganisms providing beneficial effects when consumed by humans and animals [1]. Many members of Lactobacillus spp. are consumed as health supplementary foods [2]. Are used in the food fermentation industry to ferment cereals inhibiting pathogenic bacteria [3]. Lactobacillus are gram-positive bacteria with a rod-like shape that participate in glucose fermentation. The GI tracts of diverse mammals are preferentially colonized by Lactobacillus spp. such as Lactobacillus brevis, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermentum, and Lactobacillus salivariu [6]
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