Probiotic effect of Pichia pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens

Diego Gil De Los Santos,Giana Gaboardi,Rodrigo França,Luiza Fernandes Silva,Fabricio Rochedo Conceição,Carlos Gil-Turnes,Cristina Gevehr Fernandes,João Rodrigo Gil De Los Santos,Pratyoosh Shukla

doi:10.1371/journal.pone.0192904

Diego Gil De Los Santos, Giana Gaboardi + Show 7 more

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https://doi.org/10.1371/journal.pone.0192904

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Abstract

In a previous paper we showed that the yeast Pichia pastoris X-33 grown in parboiled rice effluent supplemented with glycerol byproduct from the biodiesel industry improved the quality of the effluent. In this paper we show the validation of this yeast (PPE) as probiotic for broilers. Its effect on feed efficiency and immunomodulation was compared with the same yeast grown in yeast peptone dextrose medium (PPY), with Saccharomyces boulardii (SBY) and with the controls fed unsupplemented feed (CON). One-day-old female chicks were vaccinated against infectious bursal disease (IBD) and the titers of anti-IBD antibodies were measured by ELISA. PPE group had the highest mean titres on days 14 and 28 (p<0,05), and at 28 days, 64% of the animals showed seroconvertion. The PPE group also showed the best weight gains at 42 days of age, that, on days 7, 14 and 21 were 19%, 15%, and 8.7% higher, respectively, than the control group. The best feed conversion, 8.2% higher than the control group, was obtained by PPY at 42 days. Histopathological studies did not detect any undesirable effects in the supplemented animals. We concluded that Pichia pastoris X-33 when grown in effluents of the rice parboiling industry supplemented with glycerol byproduct from the biodiesel has probiotic properties for poultry.

Highlights

At the beginning of the century the European Community banned the use of antibiotics as growth promoters or for disease control in animal feeds [1, 2], challenging the broiler industry all around the world to find substitutes for them
In this paper we show the validation of P. pastoris X-33 grown in an effluent of the parboiling rice industry supplemented with a byproduct of the biodiesel industry as probiotic for broiler chickens in terms of safety, effect on feed efficiency and immunomodulation
This paper reports for the first time the probiotic properties in broilers of this yeast grown in the effluents of that industry

Summary

Introduction

At the beginning of the century the European Community banned the use of antibiotics as growth promoters or for disease control in animal feeds [1, 2], challenging the broiler industry all around the world to find substitutes for them. Prebiotics, probiotics and symbiotics appeared as alternatives. Several yeasts exhibited prebiotic or probiotic properties improving feed efficiency and enhancing immunity [3,4,5,6,7,8]. With a world production of 88.7 million tons in 2016, chicken meat is the second highest commodity on the worlds meat production market, being Brazil the world’s biggest exporter. Effect of P pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens decision to publish, or preparation of the manuscript

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