Abstract
In recent years, studies have focused on the therapeutic properties of probiotics to eliminate pathogenic microorganisms associated with various diseases. Lactobacilli are important probiotics groups that have been found to possess many health-promoting activities. This study was carried out to isolate LactobacillusreuteriLR12 and L. lactisLL10 from pineapple puree. The invitro analysis to evaluate probiotic characteristics of the isolated bacteria included survival in bile and acid tolerance. The cell-free supernatant of L. reuteri LR12 was effective against various pathogenic bacteria and fungi compared with L. lactisLL10. These two bacterial strains have strong anti-biofilm activity (100%) against Enterococcus faecalis, Staphylococcus aureus, and Bacillus cereus. The bacterial strains exhibited adhesion properties to HT-29 cells (human colorectal adenocarcinoma). These bacteria showed DPPH- (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical scavenging activity, scavenging of hydroxyl radical activity, superoxide radical scavenging activity, and reducing power activity in the range of 72% ± 3%to 89.3% ± 1.7%, 64% ± 2.7%to 66.8% ± 1.5%, 59.8% ± 4.1% to 63.8% ± 2.1%, and 60.4% ± 1.8%to 66.1% ± 3.3%, respectively. Pineapple puree was used as the starter culture with milk for 2 days for yogurt preparation. Pineapple puree increased flavor and showed the physicochemical properties of yogurt. The finding of the sensory evaluation revealed no significant change compared with the control, except the appearance of yogurt. These findings show that Lactobacilli and pineapple puree have potential use in various probiotic preparations for the fermentation industry.
Highlights
Intestinal microbial flora intertwines metabolic and signaling capabilities to provide various health benefits
Two probiotic organisms were isolated from pineapple for the preparation of functional food, because the presence of high sucrose and fructose is preferred for the growth of Lactobacillus sp
Probiotic bacterial strains from the genus Enterococcus were isolated from an Argentinean cheese, and their application as a starter culture for the production of cheese was suggested [32]
Summary
Intestinal microbial flora intertwines metabolic and signaling capabilities to provide various health benefits. Lactobacillus acidophilus ATCC 4356 shows strong antioxidant activity and the capability of inhibiting linoleic acid These strains survive and colonize the gastrointestinal tract of the host organism. The survival ability of probiotic organisms in the gastrointestinal tract is mainly influenced by the buffering capacity of the supplemented food. The selected bacterial strains must survive food processing and food storage conditions and withstand various stress conditions encountered in the upper gastrointestinal tract of the host organism. The pineapple fruit is an edible fruit made of flesh, with simple sugars like fructose and sucrose This fruit is rich in antioxidants (vitamin C), and consumption of this fruit is beneficial against various organisms and scavenging activity [15]. Pineapple puree was used as the starter culture for goat milk yogurt production for nutraceutical applications
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