Probiogenomics of Lactobacillus delbrueckii subsp. lactis CIDCA 133: In Silico, In Vitro, and In Vivo Approaches.

Luís Cláudio Lima De Jesus,Nina Dias Coelho-Rocha,Vasco Azevedo,Thiago De Jesus Sousa,Bertram Brenig,Rodrigo Profeta,Flávia Figueira Aburjaile,Pamela Mancha-Agresti,Mariana Martins Drumond

doi:10.3390/microorganisms9040829

Abstract

Lactobacillus delbrueckii subsp. lactis CIDCA 133 (CIDCA 133) has been reported as a potential probiotic strain, presenting immunomodulatory properties. This study investigated the possible genes and molecular mechanism involved with a probiotic profile of CIDCA 133 through a genomic approach associated with in vitro and in vivo analysis. Genomic analysis corroborates the species identification carried out by the classical microbiological method. Phenotypic assays demonstrated that the CIDCA 133 strain could survive acidic, osmotic, and thermic stresses. In addition, this strain shows antibacterial activity against Salmonella Typhimurium and presents immunostimulatory properties capable of upregulating anti-inflammatory cytokines Il10 and Tgfb1 gene expression through inhibition of Nfkb1 gene expression. These reported effects can be associated with secreted, membrane/exposed to the surface and cytoplasmic proteins, and bacteriocins-encoding genes predicted in silico. Furthermore, our results showed the genes and the possible mechanisms used by CIDCA 133 to produce their beneficial host effects and highlight its use as a probiotic microorganism.

Highlights

Lactobacillus delbrueckii subsp. lactis CIDCA 133 belongs to the culture collection of the Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA)
Specific mechanisms of action of these microorganisms have been elucidated through omics investigations [18]
L. delbrueckii CIDCA 133 has emerged as a potential probiotic strain [10,22,25]

Summary

Introduction

Many Lactobacillus strains have a probiotic profile and, present functional characteristics beneficial to the host, such as their immunomodulatory and anti-inflammatory properties [5,6], and its effectiveness on the treatment of Crohn’s disease and ulcerative colitis [7,8], intestinal mucositis [9,10], and enteric infections [11,12]. It should be emphasized that the beneficial effects of probiotics on the host are strain-dependent [13,14]

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Conclusion