Probing the structure and Franck–Condon region of protochlorophyllide a through analysis of the Raman and resonance Raman spectra

Abstract

AbstractThe structure and Franck–Condon region of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light‐regulated enzyme protochlorophyllide oxidoreductase (POR), were investigated by Raman and resonance Raman (RR) spectroscopy. The spectroscopic results are compared to the spectra of the structurally closely related porphyrin model compound magnesium octaethylporphyrin (MgOEP), and interpreted on the basis of density functional theory (DFT) calculations. It is shown that the electronic properties of the two porphyrin macrocycles are affected by different vibrational coupling modes, resulting in a higher absorption cross section of protochlorophyllide a in the visible spectral region. Furthermore, a comparison of the Fourier transform (FT)‐Raman and RR spectra of protochlorophyllide a indicates the modes that are resonantly enhanced upon excitation. Based on vibrational normal mode calculations, these modes include CC ring‐breathing and CC stretching vibrations of the porphyrin macrocycle. In particular, the strong band at 1703 cm−1 can be attributed to the CO carbonyl vibration of the cyclopentanone ring, which is attached in conjugation to the π‐electron path of the porphyrin ring system. The enhancement of that mode upon electronically resonant excitation is discussed in the light of the reaction model suggested for the photoreduction of protochlorophyllide a in the POR. Copyright © 2009 John Wiley & Sons, Ltd.