Abstract
Many dsDNA viruses, including numerous bacteriophages, utilize powerful ATP-fueled motors that repeatedly grip and translocate DNA to package viral genomes into pre-assembled capsids. Here we report preliminary results of single-molecule optical tweezers measurements of DNA gripping by the bacteriophage λ motor in different nucleotide conditions. Measurements were also made by an improved rapid solution exchange technique using a laminar, dual-solution flow cell. Similar to the behavior observed previously for the phage T4 motor, binding of g-S-ATP causes near-continuous DNA gripping and ADP causes highly intermittent gripping.
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