Abstract
As a tool, super-critical angle fluorescence (SAF) microscopy has shown tremendous promise as a means for improving the spatial resolution of TIRF microscopy and for probing real-time interactions at surfaces including non-specific surface adhesion, and peptide bonding to supported lipid bilayers (SLB). We report here on our efforts to improve and exploit the potential of SAF microscopy by exploring SAF-based anisotropy and multi-colour SAF to characterize real-time interactions at surfaces and membranes.
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