Abstract
In Reply.— Dr Datta is correct that his article1reports a probe specific forL monocytogenesusing a colony hybridization assay under less than stringent hybridization conditions. However, Dr Flamm, who initially developed and assessed the clone from which this probe was derived, reports in his thesis that the probe reacts with chromosomal DNA restriction fragments ofL monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri.2We studied the same putative β-hemolysin gene fragment cloned by Dr Flamm and showed that specific hybridization withL monocytogenesoccurs only if the hybridization is performed at highly stringent conditions. Further, we found that the fragment hybridizes to one of seven strains ofL seeligeri, even under stringent hybridization conditions.3 Subsequently, probes have been developed based on the sequence of listeriolysin that seem to be specific forL monocytogenes. We look forward to evaluation of these probes in the detection
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