Probabilistic model for estimating Listeria monocytogenes concentration in cooked meat products from presence/absence data

Abstract

A quantitative probabilistic model was developed to estimate the concentration of Listeria monocytogenes in cooked meat products based on presence/absence data and an assumed zero-inflated distribution, i.e. zero-inflated Poisson (ZIP) or zero-inflated Poisson lognormal (ZIPL) distribution. The performance of these two distributions was compared in two data sets (data set A and B), which represented L. monocytogenes prevalence and concentrations in cooked meat products. In this study, L. monocytogenes contamination data consisted of 4.23% (8/189) and 4.17% (5/120) non-zero counts for data set A and B, respectively. The contamination level of L. monocytogenes, determined by the most probable number (MPN) technique, ranged from 3 to 93 MPN/g among 13 positive samples. The goodness-of-fit test indicated that the ZIPL distribution was better than the simpler ZIP distribution, when L. monocytogenes contamination levels on positive cooked meat samples illustrated large heterogeneity. Results obtained from ZIPL distribution showed that the logarithmic mean value of L. monocytogenes positive samples was 1.5 log MPN/g (log σ = 0.4) for data set A and B. This study provides an alternative probabilistic method when only qualitative data is available in Quantitative microbial risk assessment (QMRA), in particular if pathogen concentrations consist of large numbers of zero counts and represent high variability.