Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass (Lolium Rigidum Gaud.)

Barbara Kutasy,Verëlindë Bogaj,Kinga Székvári,Syafiq A Razak,Zsolt Takács,Eszter Virág,Judit Kovács,Géza Hegedűs,Kincső Decsi

doi:10.3390/su13126648

Abstract

Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Acetohydroxyacid synthase (AHAS) resistant populations cause significant problems in cereal production; therefore, monitoring the development of AHAS resistance is widely recommended. Using next-generation sequencing (NGS), a de novo transcriptome sequencing dataset was presented to identify the complete open reading frame (ORF) of AHAS enzyme in L. rigidum and design markers to amplify fragments consisting of all of the eight resistance-conferring amino acid mutation sites. Pro197Thr, Pro197Ala, Pro197Ser, Pro197Gln, and Trp574Leu amino acid substitutions have been observed in samples. Although the Pro197Thr amino acid substitution was already described in SU and IMI resistant populations, this is the first report to reveal that the Pro197Thr in AHAS enzyme confers a high level of resistance (ED50 3.569) to pyroxsulam herbicide (Triazolopyrimidine).

Highlights

Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally
18,636,868 cleaned reads were obtained from the total raw reads of 18,642,689 (2 × 80 bp paired-end) showing Phred score 34 and GC% 55 on average
Report, sequences represented more than 0.1% of the total and low-quality bases were removed

Summary

Introduction

Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Acetohydroxyacid synthase (AHAS) resistant populations cause significant problems in cereal production; monitoring the development of AHAS resistance is widely recommended. The Pro197Thr amino acid substitution was already described in SU and IMI resistant populations, this is the first report to reveal that the Pro197Thr in AHAS enzyme confers a high level of resistance (ED50 3.569) to pyroxsulam herbicide (Triazolopyrimidine). L. rigidum is one of the most important herbicide-resistant weeds causing economic problems worldwide [6]. Since the 1980s, the development of broad and complex herbicide resistance patterns, such as multiple and cross resistance, has been detected across L. rigidum populations [7,8,9].

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