Pro-Oxidant/ Antioxidant Balance Correlates with Red Blood Cell Indices and Anemia Severity in the Anemic Patients.

Abstract

The oxidant/ antioxidant balance is disrupted in anemia. Antioxidant capacity depends on antioxidant enzyme activity and some trace elements. This study aimed to evaluate oxidant/ antioxidant status and its correlation with red blood cell indices and anemia severity in anemic patients. Blood samples were taken from 90 anemic patients and 95 healthy people. Circulatory miR-122 was assayed by real-time PCR. Malondialdehyde (MDA), pro-oxidant/ antioxidant balance (PAB), supper oxide demitasse (SOD), glutathione peroxidase (GPxs) activity, total antioxidant capacity (TAC), and zinc were measured by colorimetric method. Selenium was also determined using atomic absorption. Selenium and zinc decreased significantly in the case group (**P=0.004 and ***P=0.000). The amount of miR-122 up-regulated in the anemia (**P=0.003). MDA was significantly raised in the case vs control (***P=0.0002). PAB was higher in the case group (**P=0.005). SOD and GPxs activity was decreased along with TAC in anemic patients (*P=0.02, **P=0.008, *P=0.038). Zinc and PAB levels correlated with some red blood cell indices. PAB was associated with anemia severity. Increased PAB and decreased zinc/selenium increased oxidant levels in anemic patients. RBC indices and anemia severity were correlated with oxidant/ antioxidant somewhere.