Abstract
Dopamine increased membrane lipid peroxidation in DOPC/DOPS (8:2) liposomes which are consisting of unsaturated lipids, L-α-dioleoylphosphatidylcholine (DOPC) and L-α-dioleoylphosphatidylserine (DOPS). However, when exposed to X-rays at 4 Gy, membrane lipid peroxidation was mitigated in the presence of dopamine to the same level as an antioxidant butylated hydroxyanisole, as measured by fluorescence spectroscopy. Meanwhile, 2-phenylethylamine neither enhanced nor suppressed membrane lipid peroxidation regardless of X-ray irradiation. By the 2,2-diphenyl-1-picrylhydrazyl (DPPH)-method, dopamine showed about 4-fold the antioxidant capacity of trolox, and 2-phenylethylamine had no detectable antioxidant capacity. It was confirmed by liquid chromatography-mass spectrometry (LC-MS) analysis that the oxidation of dopamine to aminochrome was increased by X-ray irradiation at 4 Gy. These results suggest that dopamine is susceptible to autoxidation and promotes the oxidation of unsaturated lipids without X-rays, but upon X-ray irradiation, dopamine is preferentially oxidized to aminochrome together with mitigation of membrane lipid peroxidation.
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