Abstract
Cerenkov luminescence tomography (CLT) has attracted much attention because of the wide clinically-used probes and three-dimensional (3D) quantification ability. However, due to the serious morbidity of 3D optical imaging, the reconstructed images of CLT are not appreciable, especially when single-view measurements are used. Single-view CLT improves the efficiency of data acquisition. It is much consistent with the actual imaging environment of using commercial imaging system, but bringing the problem that the reconstructed results will be closer to the animal surface on the side where the single-view image is collected. To avoid this problem to the greatest extent possible, we proposed a prior compensation algorithm for CLT reconstruction based on depth calibration strategy. This method takes full account of the fact that the attenuation of light in the tissue will depend heavily on the depth of the light source as well as the distance between the light source and the detection plane. Based on this consideration, a depth calibration matrix was designed to calibrate the attenuation between the surface light flux and the density of the internal light source. The feature of the algorithm was that the depth calibration matrix directly acts on the system matrix of CLT reconstruction, rather than modifying the regularization penalty items. The validity and effectiveness of the proposed algorithm were evaluated with a numerical simulation and a mouse-based experiment, whose results illustrated that it located the radiation sources accurately by using single-view measurements.
Highlights
Cerenkov luminescence imaging (CLI) provides a potential solution to the problem of clinical translation of optical imaging, because many radionuclide probes that are widely used in clinic can be used as the light emission source of CLI
The first indicator is the distance error (Dis_Err) that defines the distance between the central position of reconstructed source and the actual center of the radionuclide probe
The second indicator is the depth error (Dep_Err) which is defined as the depth of the reconstructed source minus the depth of the actual radionuclide probe
Summary
Cerenkov luminescence imaging (CLI) provides a potential solution to the problem of clinical translation of optical imaging, because many radionuclide probes that are widely used in clinic can be used as the light emission source of CLI. CLI is a method of two-dimensional (2D) planar imaging, which cannot provide the three-dimensional (3D) spatial distribution of the radionuclide probes. It cannot accurately quantify and analyze these probes as well as the molecules they target [6]. There are several ways to reduce the ill-posedness and improve the quality of the reconstruction results, including combining the strategies of permissible source region, multi-view or multispectral measurements, and regularization terms [16,17,18,19]. Using a small amount of data to obtain accurate reconstruction results is an important problem to be solved at this stage
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