Printed-Circuit-Board (PCB) Based Multiplex Sensor for Ion Sensing

Abstract

Combining electrochemical sensing with microfluidic system presents an attractive approach for in vitro study of physiology and pathophysiology (e.g. organ-on-chip platform) [1] and for inline monitoring of environmental parameters (e.g. water quality) [2]. Ion-selective electrodes (ISE) are potentiometric sensors that measure activities of ions. They offer a simpler analytical method compare to voltammetric sensors or ISFETs. Solid-state ISEs differ from conventional ISEs in that the inner filling solution is replaced by a solid contact material. The removal of liquid compartment can make such sensor more durable and flexible, enabling it to be integrated with microfluidic device. In this work, we fabricated and evaluated a multiplex ion sensing platform by modifying the printed circuit board with solid-state ISEs. The choice of PCB platform allows straightforward multiplexing of electrodes and interfacing with downstream electronics by leveraging standardized PCB technology, which can also be cost effective in terms of fabrication. ISEs for potassium (K+) and nitrate (NO3-) approximately 1.5 mm in diameter were prepared by coating polymeric membrane and mesoporous carbon layers as the solid contact on a pre-patterned transfer tape. The electrode response was significantly stabilized by casting the membrane away from the underlying metal pads. The stabilizing effect was attributed to the increase of effective surface area of mesoporous carbon between membrane and conducting metal. The multiplex sensor was further integrated with a 3D printed microchamber for onchip analysis (Figure 1A). The K+ and NO3- electrodes displayed near-Nernstian slopes of 53 and -50 mV/dec (vs. Ag/AgCl/0.01 M Cl- reference) in KNO3 solutions, respectively. Despite some noise and baseline drift, both electrodes showed reasonable stability and reversibility upon switching solution (Figure 1B). The sensor can be stored dry between measurements. The slope of K+ electrode decayed over time, while NO3- electrode maintained sensitivity for at least one week. The slope decrease was likely due to the diffusion of ion-exchanger (tetraphenylborate) into the surrounding adhesive tape. During integration with the microchamber, the K+ electrode was also found subject to the bonding adhesive between the chamber and transfer tape, where possible reaction and/or degradation of the ion exchanger has caused complete loss of electrode response. Nevertheless, with further improvement, the PCB based multiplex ISEs provides a promising tool for ion sensing in many biomedical and environmental applications.