Abstract
This chapter introduces microbiology and basic techniques in microbiology. It gives overview of the techniques involved in culturing microorganism, including preparation and sterilization of culture media and isolation of pure cultures by aseptic techniques using the streaking method, pour-plate, spread-plate, and subculture techniques. It then explains enumeration of microorganisms by direct (direct microscopic count) and indirect methods such as standard plate count and then stains and staining techniques. By the end of the chapter methods as well as identification of bacteria through colonial characteristics, cellular morphology, biochemical procedures, serological procedures, and molecular techniques will be known. A discussion on antimicrobial susceptibility testing as well as microscope and its working mechanisms were also included.
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