Principles and applications of fluorescent probe imaging technology

Abstract

Fluorescent probe technology was discovered in the 19th century and imaging techniques were applied to microscopy around 1670. Fluorescence microscopic imaging is a classical method for observing the structure of organisms in the life sciences. In this regard, single-molecule fluorescence imaging uses fluorescent probes to label, detect, and analyze individual molecules, helping scientists to clearly observe the activities of individual molecules without disrupting the normal physiological state of the organism. In this paper, the principles and applications of fluorescent probe imaging techniques are described and analyzed. The common detection methods of fluorescent probes are spectrophotometry, electrochemistry, atomic absorption spectroscopy, high performance liquid chromatography, and fluorescence spectroscopy. Rapid detection results can be obtained depending on the specific method.