Principle and experiment of protein detection based on optical fiber sensing

Qi Jiang,Mengwei Xue,Jianqiang Lin,Jun Ouyang,Pei Liang,Chengjia Zhang

doi:10.1007/s13320-017-0383-7

Abstract

A method for detecting protein molecules based on the tilted fiber Bragg grating (TFBG) surface plasma resonance (SPR) is proposed to achieve the quick online real-time detection of trace amount of proteins. The detection principles of the TFBG-SPR protein molecular probe are analyzed, and its feasibility is demonstrated. The intermediary material between the protein molecules and the golden layer outside of the fiber gratings is cysteamine hydrochloride. When the concentration of the cysteamine hydrochloride solution is 2 M, the shift of the TFBG resonance peak is 2.23 nm, illustrating that the cysteamine hydrochloride modifies the gold film successfully. IgG antigen solution is poured on the surface of the cysteamine hydrochloride modifying the gold-deposited TFBG. Finally, antigen-antibody hybridization experiment is carried out with a 10 mg/mL antibody solution, and after two hours of hybridization the resonance peak of the TFBG shifts 5.1 nm, which validates the feasibility and effectiveness of the TFBG-SPR protein molecular probe.

Highlights

The biosensors based on the surface plasmon resonance (SPR) technology possess prominent advantages over the traditional sensors [1]
The data of cysteamine hydrochloride deposition experiment with different concentrations were taken for analysis, and the results showed that when the concentration of the cysteamine hydrochloride solution reached a certain level, the resonance peak wavelength of the tilted fiber Bragg grating (TFBG)-SPR bimolecular sensor shifted to the larger ones, and the cysteamine hydrochloride deposition was successful
This paper proposes a method to detect protein molecules based on the TFBG-SPR principles and demonstrates the feasibility by experiments

Summary

Introduction

The biosensors based on the surface plasmon resonance (SPR) technology possess prominent advantages over the traditional sensors [1]. The SPR technology, as a new molecular detecting technology, had got a rapid development in recent years. The number of documents in the relevant field has a rapid increase with the vigorous development of the SPR sensor based on the optical fiber and the advent of related products [2]. These years, the SPR sensors have been paid more attention in the field of molecular detecting [3, 4].

Methods

Results

Conclusion