Principal component analysis of interrelations in stored‐wheat ecosystems infested with multiple species of insects

Abstract

SummaryPrincipal component analysis (PCA) was used to determine the effects of infestation of bulk stored wheat by multiple species of insects at 30±2°C for 60 wk. Eight 204‐liter drums containing wheat at 15.5% moisture content were used as three distinct man‐made ecosystems: (a) Control system (2 drums), insect‐free; (b) RST system (3 drums), artificially infested with Rhyzopertha dominica (F.),Sitophilus oryzae (L.), and Tribolium castaneum (Herbst); and (c) COT system (3 drums), infested with Cryptolestes ferrugineus (Stephens),Oryzaephilus surinamensis (L.), and Tribolium castaneum. The variables measured tri‐weekly within each system included carbon dioxide, oxygen, temperature, grain moisture, seed damage, grain weight and volume, dust weight and volume, fat acidity values (FAV) of the wheat, seed germination, microflora including Alternaria alternata(Fr.) Keissler,Aspergillus glaucus group, Aspergillus candidusLink, and bacteria, insects and the mite Tarsonemus granariusLindquist. PCA provided multivariate synopsis of the data quantifying several important relationships among the variables monitored. Tri‐weekly and cumulative 60‐wk analyses of each system showed that high bacterial counts were associated with high FAV levels;Tarsonemus numbers were positively related to Aspergillus; Alternaria and seed germination were negatively related to FAV, bacteria and grain damage; and that the number of insects was related to the presence of Aspergillus and negatively related to the presence of bacteria. Seed germination and Alternaria infection often decrease rapidly presumably because of infection by fungi of the Aspergillus glaucus group. The combined action of R. dominica and Aspergillus spp. enhanced seed damage and increased grain moisture content thus promoting bacterial growth which in turn inhibited insect and mold growth. Fat acidity values increased with time unless seed damage and bacterial infection were extensive as in the RST system.