Abstract
BackgroundPrimulina cardaminifolia Yan Liu & W.B. Xu (Gesneriaceae), a distinct new species with imparipinnate leaves, is described and illustrated from a limestone valley in Guangxi Zhuangzu Autonomous Region, China. To assure its generic placement and phylogenetic affinity, phylogenetic analyses were performed using DNA sequences of nuclear ITS and chloroplast trnL-F intron spacer region. Additionally, somatic chromosome number was counted and pollen stainability was tested.ResultsPhylogenetic analyses support its placement in Primulina; however, two phylogenetically distinct ITS sequence types were detected, suggesting a probable hybrid origin. Its pollen stainability is 100% and its chromosome number, 2n = 36, is congruent with all known counts of diploid species of the genus.ConclusionAll available data support the recognition of the new species Primulina cardaminifolia and suggest that it could have derived from homoploid hybrid speciation. Color plates, line drawings and a distribution map are provided to aid in identification.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-54-19) contains supplementary material, which is available to authorized users.
Highlights
Classification of chromosome complement based on centromere position at mitotic metaphase followed Levan et al (1964)
Molecular methods DNA sequences of the nuclear ribosomal internal transcribed spacers (ITS) and the chloroplast trnL-F intron spacer region were gathered using protocols outlined in Xu et al (2012a)
Following the manufacturer’s protocol, the purified ITS templates were ligated to the pGEMT-T vector system (Promega, Madison, Wisconsin, USA) and subsequently transformed into competent cells (DH5α) to perform molecular cloning
Summary
Primulina cardaminifolia Yan Liu & W.B. Xu (Gesneriaceae), a distinct new species with imparipinnate leaves, is described and illustrated from a limestone valley in Guangxi Zhuangzu Autonomous Region, China. With over 130 species transferred to (Wang et al 2011; Weber et al 2011; Xu et al 2012b) and more than ten species newly described (Liu et al 2011; Hong et al 2012; Huang et al 2012; Li et al 2012; Wen et al 2012a, b, c; Wu et al 2012a, 2012b; Xu et al 2012a; Chung et al 2013), Primulina has become one of the largest
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