Abstract
Gonadal development is a sequential process that can be divided into three major events: the PGCs migration, sex determination and gonadal differentiation. This study was aimed to see the development of PGCs isolated from the gonads of embryos after being incubated for 7 days and then was incubated using a solution of Phosphate Buffer Saline (PBS) [-]. The developing gonad can be isolated from 7 days old chick and can be incubated at a temperature of 37.8<sup>o</sup>C in a solution of PBS [-]: without Ca2+ and Mg2+. The release of gonadal PGC was observed within 1, 8, 16, and 24 hours after the embryonic gonad was placed in a PBS solution [-]. The results showed that PGCs can be separated from gonadal tissues and can be collected by entering the developing gonad to the PBS [-] solution. The highest percentage of PGCs and survival rate was obtained after gonad was incubated for 1 hour and was not different with 8 hours (P&gt;0.05). Those result was significantly different (P&lt;0.05) with the 16 and 24 hours incubation. The highest purity rate percentage was in the 8 hours incubation, but did not show a significant difference (P&gt;0.05) with the 1 and 16 hours incubation. The percentage of the purity differed (P&lt;0.05) after the 24 hours incubation. It can be concluded that the most appropriate incubation time to obtain PGCs from the KUB chicken embryonic gonad is no more than 8 hours.
Highlights
Primordial germ cell (PGC) are the precursors of the progenitors of the oocytes and spermatocytes, or according to D'Costa et al (2001), PGC are the precursors of mature ovum and spermatozoa of the mature poultry and an intergenerational genetic linkage
The results showed that PGCs can be separated from gonadal tissues and can be collected by entering the developing gonad to the Phosphat Bufer Saline (PBS) [-] solution
Primordial germ cells profiles incubated for 24 hours in phosphate buffer saline [-] solution identical (Park & Han 2012), the advantage of using gonadal PGcs compared with another sources of these cells is, that a greater number of PGcs can be retrieved from one embryo. (Bednarczyk 2014)
Summary
Primordial germ cell (PGC) are the precursors of the progenitors of the oocytes and spermatocytes, or according to D'Costa et al (2001), PGC are the precursors of mature ovum and spermatozoa of the mature poultry and an intergenerational genetic linkage. In many animal species, including birds, germ cells emerge from a small population of cells known as PGC (Bednarczyk 2014). Primordial germ cells profiles incubated for 24 hours in phosphate buffer saline [-] solution identical (Park & Han 2012), the advantage of using gonadal PGcs compared with another sources of these cells is, that a greater number of PGcs can be retrieved from one embryo. Primordial germ cells profiles incubated for 24 hours in phosphate buffer saline [-] solution identical (Park & Han 2012), the advantage of using gonadal PGcs compared with another sources of these cells is, that a greater number of PGcs can be retrieved from one embryo. (Bednarczyk 2014)
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