Abstract
Recently DNA polymerases of eukaryotes have been the subject of intensive investigation as a consequence of the importance of research on RNAdependent DNA polymerases in normal [ 1, 21 and in tumor cells and tissues [3-61. Attention has also been focused on the activity of DNA polymerases in regenerating tissues [7] and in developing systems [8-l 11. Margolis [ 121 has studied the distribution patterns of DNA-dependent DNA polymerase in different regions of brain of chick embryo, and one of us has shown [ 131 that mitochondria and nuclei of chick embryonic brain contain bound enzymes which catalyze the polymerization of dGMP when poly dG:poly dC or poly dG:poly rC are provided as primer templates. In the present paper we report the characterization of partially purified poly dT:poly rA-dependent DNA polymerase (bound to nuclei) and oligo dT:poly rAdependent DNA polymerase (bound to mitochondria), both of them polymerizing thymidylic acid. Moreover, we have studied poly dG:poly dC and poly dG:poly rC-dependent activities polymerizing deoxyguanylic acid, in both mitochondria and nuclei.
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