PRIMER NOTE: Isolation and characterization of microsatellite loci in the Pacific pleasure oyster, Crassostrea corteziensis, and their cross-species amplification in four other oyster species

Abstract

Microsatellite loci were isolated in Crassostrea corteziensis using (GT)n, (CT)n and (CTGT)n-enriched genomic libraries. Within each of 45 sequenced clones, an average of three microsatellite regions (156 total) were observed. Thirty-three primers were designed, from which 11 microsatellite loci amplified. Ten of those were polymorphic, with a range of two to 30 alleles. Three loci were not in Hardy–Weinberg equilibrium, and linkage disequilibrium was found for six pairs of loci. These microsatellite loci will be further tested for segregation distortions and null alleles to establish a set for population genetic studies of the species in the Northwest coasts of Mexico, and for optimization of aquaculture development. Seven of the microsatellite loci cross-amplified in Crassostrea palmula, a sympatric species, and will be useful in further genetic studies.