Prime indagini sul comportamento di embrioni isolati diTriticum DurumCv. « Cappelli », provenienti da cariossidi normali e bianconate allevati artificialmente

Abstract

Abstract SOME INVESTIGATIONS ON THE BEHAVIOUR OF EXCISED EMBRYOS OF TRITICUM DURUM CV. « CAPPELLI », COMING FROM NORMAL GRAINS AND YELLOW BERRIES. — Excised embryos of Triticum durum cv. « Cappelli », coming from normal grains and yellow berries, soaking in distilled water for 24 hours, were brought up for 6 days in petri dishes, on a 1% solution of agar + glucose, at the constant temperature of 23°C, at the light of a common germicidal lamp. As controls, normal grains and yellow berries were utilized, placed in petri dishes each containing a piece of filter paper moistened with distilled water. After 6 days, the seedlings (measured the shoot and the longest root) were transferred to a greenhouse in sand, at daylight (average temperature: 16°-18°G.). After 14 days, the shoot and the longest root of each seedling coming from the sand cultures were measured. The following results were obtained: control seedlings: after 7 (1 + 6) days, the normal seedlings grow more than those coming from yellow berries and the shoots grow as the roots (see MELETTI e ONNIS 1961 a, b e c); after 21 days, the situation of the control seedlings is again completely regular (normal shoots are longer); seedlings coming from excised embryos: differences of growth are not here, neither after 7 nor after 21 days, among seedlings coming from normal grains and yellow berries. These data should allow to conclude that the embryos, coming from normal grains and yellow berries, grow in the same way. But the limits of the utilized technique (want of balance in the relations between shoot and root, low survival of the seedlings coming from the excised em bryos, which grow too little in respect of the controls) suggest to carry into other investigations on the same subject.