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Abstract
The mucins isolated from Rana temporaria egg jelly coats were found to be composed of Gal, Fuc, GlcNAc, Ga1NAc and GlcA acid. The primary structure of seven sulfated oligosaccharide-alditols was obtained by 1D/2D NMR analyses ( 1H- 13C). The results show the presence of the sulfated monosaccharides HSO 3(3)Gal, HSO 3(6)GlcNAc and HSO 3(3)GlcA. The sequence HSO 3(3)GlcA(β1–3)Gal, which constitutes the major determinant of the HNK-1 oncofoetal epitope, was characterized.
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