Abstract
The amino acid sequences have been determined for two CNBr fragments of human alpha 2-macroglobulin which, due to the presence of an uncleaved Hse-Thr bond, form an Mr = 40,000 fragment. These fragments are located in the COOH-terminal part of alpha 2-macroglobulin (CB21, residues 955-1185 and CB22, residues 1186-1291). CB21 contains one glucosamine-based carbohydrate group attached to Asn-14 and one internal disulfide bridge (Cys-102 bound to Cys-150). CB21 and CB22 account for 337 of the 1451 residues of the subunit of alpha 2-macroglobulin.
Highlights
Peptide Nomenclature bond, forman M, = 40,000 fragment
It SequenceDetermination (Fig. 1)-Following reduction, carwas found that one of these sets produced a complex set of boxymethylation, and gel chromatography of the M, = 40,000 fragments ( M r = 40,000 to M, < 10,000)whichcouldbe fragment sets found in pool 1C (Fig. 7 (I)), a fraction was partially separated bygel chromatography
A detailed description of the materials and methods used and of These results indicated that the M, = 40,000 fragment conthe purification of the CNBr fragments is presented in a preceding sisted of CB21 connected to CB22 byan uncleaved -Hse-Thrarticle (1)
Summary
The analysis of the weak overlap between T4 and T5 by C7 (and C9) were this mixturewas facilitated by the previous determination of settled by the results of sequenator analysis of SP3 region [43-70] by T5, T6,C20, P3, C13, and TK1, anodf region (Table 10). C12 was pure and apparently contained [111-140] by C20, C21, “TSP,”and hydroxylamine-cleaved residues [56-64], no completesequence of thispeptide was CB21+22. The identification of -ValGs-Leu-Lysin partwas the outcome of the analysisof a mixture of peptides, thelocation and TK1confirmed the sequence for the first 40 residues of of this stretch and the stretch-Val'sg-Leu-Lys was judged to CB22 determined by sequenator analysis. This does not invalidate the assignment of the minal 17 residues of CB22 was determined via T11, C8, C9, disulfide bridge in CB21, since the peptide Leu-Asn-Ser-His- and P5. As discussed in Refs. 1and 5 , the pool 1C material was contaminatedwith the medium-sized fragments found inpool 2,3C
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
