Primary Spinal Cord Neurons Better Attach to Non-Coated Surfaces Than to Poly(D-lysine)-Coated Surfaces

Abstract

Background: Cell culture is an important technique in cellular and molecular biology. There are two basic systems for culturing cells, adherent (monolayer) culture and suspension culture. In adherent cell culture, cellular adhesion molecules play a vital role in many physiological processes. Many cells, especially primary-obtained cells, hardly attach to plates, so coating agents are used for cell attachment and growth. There is no striking report on whether all cells need coated plates. Objectives: We investigated whether a coating plate is essential for primary spinal cord-obtained neuron culture. Methods: Cells were separated and seeded in tissue culture plates coated with either Poly(L-lysine) or Poly(D-lysine), as well as without coating containing DMEM-F12 media with 10% FBS. Results: Interestingly, we found that neuron cells more potently attached to coating free plates. Conclusions: These results provide strong evidence helping the researchers to optimize primary spinal cord cultures.