Primary recovery of alkaline phosphatase from hepatopancreatic tissues of Indian white shrimp at varied centrifugal force and time

Abstract

Hepatopancreatic tissues of Indian white shrimp (Penaeus indicus) homogenate was centrifuged at relative centrifugal force (RCF) of 67.2, 1,681.1, 6,724.3, 15,124.8, or 26,897.4×g for 5, 10, 15, or 20 min at 4°C. Impact of centrifugal force and time on the primary clarification and alkaline phosphatase activity of the homogenate was determined by the proportionate at which solids, proteins, lipids, and alkaline phosphatase fractioned inbetween supernatant, infranatant, and pellets. Optimum yield of the enzyme achieved at RCF of 1,681.1×g for 5 min at 4°C was 90.28±1.55%. The forces below this level even upto 20 min removed only up to 56.57±0.66% of the total solids and 7.90±0.19% of the total lipids from the homogenate. At the force and time above RCF of 1,681.1×g and 5 min, specific activity was increased by 4.18±0.38-fold due to the loss of total protein, but upto 60.48±2.61% of alkaline phosphatase activity was lost.