Primary in vivo Immunization of F1 (2 × 13) Guinea Pigs by Parental Macrophages Bearing Multideterminant and Genetically Restricted Antigens

Abstract

Abstract An objection has been raised to the concept of macrophage Ir gene function by the in vivo experiments of Pierce et al. in which nonresponder murine macrophages (Mϕ) primed responder mice, thus suggesting Ir gene function at the level of the T cell. Both to examine this issue in the guinea pig and to determine whether in vitro secondary responses are solely reflections of the animal's initial immunologic experience, inbred strain 2 and 13 guinea pigs were immunized in vivo with syngeneic or allogeneic antigen bearing Mϕ. Monolayer purified Mϕ were pulsed with oxidized B chain of insulin and dinitrophenylated ovalbumin (DNP-OVA) for 60 minutes, washed and 2 × 107 injected intradermally into recipient animals. Fourteen days later, in vitro antigen induced specific T cell proliferation was assessed. When strain 13 guinea pigs, high responder to both antigens, were immunized by antigen pulsed strain 13 Mϕ, T cells proliferated in vitro to both antigens.