Abstract
A method is described for the isolation and culture of neurons in the adult mammalian brain. The cell could be maintained in primary culture for more than several weeks. Whereas the neurons freshly dissociated from the adult brain did not respond to any of the neurotransmitter substances applied, the neurons regained the ability to respond to a variety of neurotransmitters when cultured. The cultured neurons of the adult mammalian brain may be an excellent model for physiological as well as pharmacological investigations of the central nervous system.
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