Abstract
The alteration of the mucociliary clearance is a major hallmark of respiratory diseases related to structural and functional cilia abnormalities such as chronic obstructive pulmonary diseases (COPD), asthma and cystic fibrosis. Primary cilia and motile cilia are the two principal organelles involved in the control of cell fate in the airways. We tested the effect of primary cilia removal in the establishment of a fully differentiated respiratory epithelium. Epithelial barrier integrity was not altered while multiciliated cells were decreased and mucous‐secreting cells were increased. Primary cilia homeostasis is therefore paramount for airway epithelial cell differentiation. Primary cilia‐associated pathophysiologic implications require further investigations in the context of respiratory diseases.
Highlights
Most primary cilia (PC) studies in respiratory research are restricted to development or repair processes
These solitary non-mobile organelles act as sensors and molecular signalling hubs while motile cilia (MC) in differentiated epithelial cells are paramount for mucociliary clearance.[1]
As it was suggested that multiciliated cells (MCC) originated from primary ciliated cells (PCC),[6] we investigated the consequences of PC removal on human airway epithelial cell (AEC) differentiation
Summary
Most primary cilia (PC) studies in respiratory research are restricted to development or repair processes. These solitary non-mobile organelles act as sensors and molecular signalling hubs while motile cilia (MC) in differentiated epithelial cells are paramount for mucociliary clearance.[1] We recently demonstrated the presence of PC in human airway epithelial undifferentiated cells during homeostasis and in chronic obstructive pulmonary disease (COPD).[2] In addition, recent studies reported a decrease in multiciliated cells (MCC) in COPD patient-d erived bronchial and bronchiolar air-liquid interface (ALI) cultures,[3,4] and we highlighted an abnormal cilia-associated. As it was suggested that multiciliated cells (MCC) originated from primary ciliated cells (PCC),[6] we investigated the consequences of PC removal on human airway epithelial cell (AEC) differentiation
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