Abstract
A comprehensive assay of proliferative and hormonal activity in primary cell cultures derived from neonatal pig thyroid was carried out for the first time. Morphology and basal and thyroid-stimulating hormone (TSH)-stimulated secretion of thyroxin were evaluated in cultures, depending on the initial material placed into the culture: single cells or follicular conglomerates. Spontaneous and under chronic TSH stimulation folliculogenesis and formation of dome structures were assessed in culture. It was demonstrated that the cells expressed β-III-tubulin during prolonged cultivation with nerve growth factor.
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