Preventive Study Garlic Extract Water (Allium sativum) Toward SGPT, SGOT, and the Description of Liver Histopathology on Rat (Rattus norvegicus), which were exposed by Rhodamine B

Abstract

Rhodamine B is a textile coloring materials which often mixed in food coloring. The use of Rhodamine B in food for a long time may result in liver dysfunction or cancer. However, when exposed Rhodamine B in large quantities in a short time it will be symptoms of acute poisoning of Rhodamine B. The allicin and alliin content in garlic works as an antioxidant that can neutralize free radicals there by lowering oxidative stress and help prevent an increase in SGOT and SGPT and improve depiction hepatic histopathology. The purpose of this study are to determine the effect of preventive therapy extract water of garlic (Allium sativum) on levels of SGOT and the SGPT and liver histopathology description of rat (Rattus norvegicus) which were exposed by Rhodamine B. This study used male rats strain Wistar with 8 weeks of age and weight of 200 grams which were divided into 5 groups: group A (negative control), B (positive control), group C, D, and E were fed with rodhamin B and given preventive water with garlic juice with successive doses of 0.5 mL, 1 mL, and 1.5 mL. SGPT and SGOT level measurements performed by spectrophotometric method and observation of rat liver histopathology performed using light microscope. The data analysis activities of SGPT/SGOT using ANOVA, and the description of histopathology were analyzed descriptively. The results showed giving garlic extract with a dose of 1.5 mL/0.2 kg bw were able to decrease the activity of SGPT, i.e. respectively for treatments A to E, 18.89%, 17.3 %, 40.96% and SGOT 41.44%, 45.96%, 49.69%, and to improve the hepatocyte cells in rat exposed to Rhodamine B. The conclusion of this study is water garlic juice can be used as herbal therapy in mice which were exposed by Rhodamine B.