Abstract
Purpose: The present study was intended to investigate the preventive role of Withania somnifera (WS) on hyperlipidemia and oxidative stress in the heart of streptozotocin (STZ)-induced type 2 diabetic rats.Methods: Single intraperitoneal injection of STZ (100 mg/kg) was given to 2 days rat pups to induce type 2 diabetes mellitus. Diabetes was confirmed 90 days after the administration of STZ by measuring blood glucose level. WS (200 and 400 mg/kg) was administered orally once a day for 5 weeks after the confirmation of diabetes. Glucose, lactate dehydrogenase (LDH), creatinine kinase (CK), total cholesterol (TCh), triglycerides (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), verylow density lipoprotein cholesterol (VLDL-C) and markers of oxidative stress parameters like lipid peroxidation (LPO), reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) were evaluated in the heart of type 2 diabetic rats.Results: Oral administration of WS for 5 weeks resulted in a significant (P<0.001) reduction in glucose, LDH, CK, TC, TG, LDL-C, VLDL-C levels with significant elevation of HDL-C levels. On the other hand, WS treated diabetic rats significantly (P<0.01-P<0.001) reduced the elevated levels of LPO, increased levels of antioxidant enzymes (i.e, GSH, GPx, GR, GST, SOD and CAT).Conclusion: These findings propose the role of hyperlipidemia and cardiac oxidative stress in type 2 diabetic rats and suggested protective effect of WS in this animal model.Keywords: Withania somnifera; Hyperlipidemia; Oxidative stress; Streptozotocin; Type 2 diabetes
Highlights
Type 2 diabetes mellitus (DM) is one of the most common problems for the health care system, under which most of the patients with this disease are treated
For several years it has been suggested that patients with diabetes experience chronic oxidative stress which results from disturbance in the balance between the formation of free radicals in the body and scavenging of these radicals [3]
Oral administration of Withania somnifera (WS) revealed that the both doses (200 and 400mg/kg) significantly (P
Summary
Type 2 diabetes mellitus (DM) is one of the most common problems for the health care system, under which most of the patients with this disease are treated. For several years it has been suggested that patients with diabetes experience chronic oxidative stress which results from disturbance in the balance between the formation of free radicals in the body and scavenging of these radicals [3]. We have published the protective effect of Withania somnifera against oxidative stress and pancreatic β-cell damage in type 2 diabetic rats [11]. The present study was planned to investigate the preventive effect of WS on hyperlipidemia and oxidative stress in the heart of type 2 diabetic rats. Single intraperitoneal (i.p) injection of STZ (100 mg/kg) was given to 2 days old rat pups to induce type 2 diabetes mellitus [13]. Type 2 diabetes mellitus was confirmed 90 days after the administration of STZ by measuring blood glucose level.
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