Abstract
Saccharomyces cerevisiae was used as a model to explore the preventive effect of two marine polysaccharides separately derived from Sepia esculenta ink (SIP) and Laminaria japonica (FL) as well as one terrestrial polysaccharides from Eleocharis tuberosa peel (WCPP) on toxic injury induced by acrylamide (AA). The growth of yeast was evaluated by kinetics indexes including doubling time, lag phase and maximum proliferation density. Meanwhile, intracellular redox state was determined by contents of MDA and GSH, and SOD activity. The results showed that AA inhibited yeast growth and destroyed the antioxidant defense system. Supplement with polysaccharides, the oxidative damage of cells was alleviated. According to the growth recovery of yeast, FL and WCPP had similar degree of capacity against AA associated cytotoxicity, while SIP was 1.5~2 folds as strong as FL and WCPP. SIP and FL significantly reduced production of MDA by AA administration. Moreover, SIP, FL and WCPP increased SOD activity and repressed GSH depletion caused by AA.
Highlights
Acrylamide (AA) is a monomeric chemical that can be used as polymeric flocculant in textile and paper industry after forming polyacrylamide [1]
One of the important toxicological mechanisms is that AA mediates intracellular oxidative stress by promoting generation of reactive oxygen species (ROS), leading to the depletion of glutathione (GSH), and consequent DNA damage [5,6]
Since it is recognized that AA is widely derived from the diet during high temperature processing, the harmful effect of AA on human health has been well-studied [19]
Summary
Acrylamide (AA) is a monomeric chemical that can be used as polymeric flocculant in textile and paper industry after forming polyacrylamide [1]. One of the important toxicological mechanisms is that AA mediates intracellular oxidative stress by promoting generation of reactive oxygen species (ROS), leading to the depletion of glutathione (GSH), and consequent DNA damage [5,6]. In recent years, maintaining the redox equilibrium in tissues or cells with natural or synthetic active compounds has become a research hotspot, which was considered as a potential treatment strategy for diseases caused by oxidative stress [7,8,9]. Among these active compounds, natural polysaccharides have a wide range of beneficially therapeutic effects and health-promoting properties. As antioxidants in vivo and in vitro, polysaccharides can effectively scavenge free radicals and protect organisms from oxidative damage [10]
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