Preventive effects of the antioxidant and antigenotoxicAchyrocline satureioides extract against zearalenone-induced mammal cytogenotoxicity and histological damage

Abstract

Zearalenone (ZEN), aFusarium’s mycotoxin, is immunotoxic, genotoxic, hepatonephrotoxic and, affects the reproductive system. ZEN induces toxic and genotoxic effects on humans and other animals.Achyrocline satureioides has several medicinal properties. Moreover, the aqueous extract ofA. satureioides is a safe agent that exerts low cytotoxicity and no genotoxicity. This extract is a promissory candidate to counteract ZEN effects. The present study aimed to investigate the capacity of cold aqueous extract fromA. satureioides to protect against ZEN multi-target toxicity in different experimental mammal models. Anticytotoxicity was evaluated by neutral red uptake and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium reduction assays. Comet assay and micronuclei test, oxidative stress (TBARs), and histopathological damage were evaluated in Balb/C mice. Anticytotoxic studies indicated that cold aqueous extract (100 and 300 μg/ml) protected from damage induced by ZEN (50 μg/ml) on Vero cells.In vivo studies indicated that ZEN (40 mg/kg body weight) induced an increase of genotoxicity: micronuclei (34 MNPCE/1000 PCE) and increase of damage (tail moment) in blood cells. Also, it increased lipid peroxidation in liver and kidneys and generated several histopathological alterations in both organs. Cold aqueous extract (100 mg/kg body weight) protected from genotoxicity induced by ZEN in both tests. Cold aqueous extract, also, reduced the lipid peroxidation and histopathological damage in liver and kidneys. In conclusion, the cold aqueous extract ofA. satureioides that contains bioactive flavonoids prevents the multi-target toxicity induced by ZEN improving all the parameters evaluatedin vitro andin vivo, which is a valuable and original finding in order to develop future treatments for human and veterinary medicine.