Preventive Effects of Health-Food Drinks on the Obesity and DNA Damage of Mice Fed a High-Fat Diet under a Mild Stress

Minoru Higashimoto,Arinobu Yamauchi,Nao Tokumoto,Chikako Toda

doi:10.6000/1929-4247.2016.05.03.3

Minoru Higashimoto, Arinobu Yamauchi + Show 2 more

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https://doi.org/10.6000/1929-4247.2016.05.03.3

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Abstract

The body weight gain of mice fed an obesity-inducing diet is suppressed by a mild restraint stress, but at the same time, as previously reported, the stress induces DNA damage in the cells of multiple organs. In the present study, we attempted to prevent not only the obesity but also the DNA damage of mice fed an obesity-inducing diet under a mild restraint with commercially available health-food drinks such as fruit and vegetable juices, soymilks, vinegars and lactic acid bacteria drinks, which are rich in antioxidants. The body weight gain of young female mice fed a high-fat diet containing 20% fat for 4 weeks was considerably inhibited by restraint for 15 min per day in weeks 2 to 4 of 4-week period. The inhibition was further promoted with the concomitant administration of health-food drinks noted above, and was accompanied by a decrease of periovular fat, a major abdominal fat in the female mice. The definite loss of energy intake in the mice given health-food drinks was approximately compensated by the energy of the drink administered. On the other hand, the increase of DNA damage generated by restraint in the cells of five organs – the liver, pancreas, spleen, heart and bone marrow – was markedly suppressed with the administration of these drinks. The results suggest that a combination of mild stress and intake of suitable health-food containing some antioxidants may inhibit lifestyle-related diseases including hyperlipidemia and obesity, which may contribute to the inhibition of metabolic syndrome and childhood obesity.

Highlights

It has been shown that mental and physiological stresses induce excess reactive oxygen species (ROS), worsen lifestyle-related diseases, cause cellular damage via oxidation of biomolecules, including DNA, proteins and polyunsaturated fatty acids, and induce a variety of diseases such as cancer, Parkinson’s disease, and cardiovascular diseases [14]
Data of the control groups (C1, C2, F12, F24 and R15) and the experimental drink groups are presented as means obtained from two experimental groups for twelve fruit and vegetable juice groups (FV-A: Fv1-5 and FV-B: Fv6-12), n=12
Childhood obesity, which is associated with metabolic syndrome, diabetes, heart disease and cancer, is one of the most debilitating and serious public health issues worldwide [4, 8, 9]

Summary

Introduction

It has been shown that mental and physiological stresses induce excess reactive oxygen species (ROS), worsen lifestyle-related diseases, cause cellular damage via oxidation of biomolecules, including DNA, proteins and polyunsaturated fatty acids, and induce a variety of diseases such as cancer, Parkinson’s disease, and cardiovascular diseases [14]. We previously reported that foodinduced dyslipidemia in mice could be accelerated by repeated fasting, but could be suppressed by repeated restraint, and that the DNA damage in cells of various organs was increased by repeated stress [13, 15]. In these studies, we investigated the DNA damage in the EXPERIMENTAL METHODS. Control mice were fed standard diet (STD) (C1) or HFD (C2) for 4 weeks

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