Abstract
We recently reported that the intraperitoneal inoculation of Lactobacillus helveticus SBT2171 inhibited the development of collagen-induced arthritis (CIA), a murine model of rheumatoid arthritis (RA). In the present study, we evaluated the effect of the oral administration of L. helveticus SBT2171 on CIA development and on the regulation of antigen-specific antibody production and inflammatory immune cells, which have been implicated in the development of RA. Both oral administration and intraperitoneal inoculation of L. helveticus SBT2171 reduced joint swelling, body weight loss, and the serum level of bovine type II collagen (CII)-specific antibodies in the CIA mouse model. The intraperitoneal inoculation also decreased the arthritis incidence, joint damage, and serum level of interleukin (IL)-6. In addition, the numbers of total immune cells, total B cells, germinal center B cells, and CD4+ T cells in the draining lymph nodes were decreased following intraperitoneal inoculation of L. helveticus SBT2171. These findings demonstrate the ability of L. helveticus SBT2171 to downregulate the abundance of immune cells and the subsequent production of CII-specific antibodies and IL-6, thereby suppressing the CIA symptoms, indicating its potential for use in the prevention of RA.
Highlights
Lactic acid bacteria (LAB) is a general term used to represent bacteria that produce lactic acid by metabolism
We examined the efficacy of the intraperitoneal inoculation and oral administration of L. helveticus SBT2171 to attenuate collagen-induced arthritis (CIA) in mice
The intraperitoneal inoculation of L. helveticus SBT2171 suppressed the incidence of arthritis and the increase of clinical score, and decreased body weight loss and hind paw thickness when compared to the control CIA mice (Figures 2, 3A,B)
Summary
Lactic acid bacteria (LAB) is a general term used to represent bacteria that produce lactic acid by metabolism. There are relatively few reports on the immunepromoting properties of LAB, they have been shown to both downregulate and activate components of the immune system. These reports have demonstrated that LAB have an alleviative effect on allergy (Shandilya et al, 2016; Yamamoto et al, 2016) and autoimmune diseases (Kato et al, 1998; So et al, 2008; Amdekar et al, 2011) resulting from an excessive immune system response due to mistaking originally harmless triggers such as food, pollen, and self-proteins as harmful. The specific mechanisms by which LAB exert these effects remain unclear
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