Preventive Effect and Molecular Mechanism of Lactobacillus rhamnosus JL1 on Food-Borne Obesity in Mice.

Mo Yang,Xinyan Yang,Jiapeng Zheng,Yu Zhang,Chaoxin Man,Yujun Jiang,Xinran Zong

doi:10.3390/nu13113989

Abstract

Probiotics can prevent obesity and related metabolic complications. In our study, the protective effect and molecular mechanism of Lactobacillus rhamnosus JL1 (separated from the feces of healthy infants) on high-fat diet mice were investigated. After 10 weeks of dietary intervention with L. rhamnosus JL1 intervention, the body weight of the JL1 group (23.78 g) was significantly lower than that of the HFD group (26.59 g, p < 0.05) and the liver index was reduced. Serum biochemical analysis showed that the TC, TG and LDL-C contents of JL1 group mice were significantly decreased (p < 0.05). Histological images of the mice livers showed that the degree of lipid action and damage of hepatic cells were improved. L. rhamnosus JL1 activated the AMPK pathway, and reduced the gene expression of PPAR-γ, LXR-α and SREBP-1C. In addition, the protein expression of PPAR-γ and LXR-α were reduced. After dietary intervention with L. rhamnosus JL1, the concentration of acetic acid, propionic acid, and butyric acid were increased significantly, especially the concentration of butyric acid, which was 63.16% higher than that of the HFD group (p < 0.05). In conclusion, this study provided a theoretical reference for the development and application of probiotics derived from healthy infant feces in health products and functional foods.

Highlights

Probiotics may be able to alleviate obesity caused by high-fat and high-sugar diets; they are a potential solution for the treatment of lipid metabolism disorders caused by obesity in recent years
L. rhamnosus JL1 had a good effect on reducing blood lipids and lowering weight
After 10 weeks of diet intervention, the body weights of the JL1 group were lower than those of HFD group, indicating that L. rhamnosus

Summary

Introduction

Chronic diseases such as tumours and diabetes are all related to obesity. The prevention and treatment of metabolic diseases must be fully considered in their association with obesity [1]. Epidemiological evidence has confirmed that high-fat diets are closely associated with increased susceptibility to diseases such as hypertension, hyperlipidemia, and atherosclerosis [2]. Effective prevention of obesity is vital to reduce the occurrence of cardiovascular and cerebrovascular diseases. Medicines and surgery may bring adverse effects, so it is vital to develop healthy long-term eating habits [5]. Probiotics may be able to alleviate obesity caused by high-fat and high-sugar diets; they are a potential solution for the treatment of lipid metabolism disorders caused by obesity in recent years

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Conclusion