Abstract
Materials and methodsIn this study, 10 male Shall sheep were used in two groups and bone marrow samples were collected and BM-MSCs isolated. Then experimental model of ARDS was induced by intrapulmonary injection of LPS to dose of 400 μg/kg. Twenty-four hours after LPS injection, 5 × 107 cells of BM-MSCs were autologous transferred in the group of treatment and 1 ml PBS was infused in the group of control as intrapulmonary. Then, the symptoms of clinical, complete blood count, analysis of arterial blood gases and the concentrations of IL6,IL10,TNF-α,total protein, Ig M and albumin BAL were determined before and at times of 3,6,12,24,48,72, and 168 after transplantation/infusion. Key findingsThe results of the investigations 24 h post-LPS injection(time 0) indicated the occurrence of acute inflammation which confirmed ARDS model. These changes included increase in RR, HR and RT, decrease in PO2 and SatO2 and increase in PCO2, WBC, neutrophils, macrophages, total protein,IL6,IL10, TNF-α,Ig M and albumin. But the stem/stromal cells transplantation reduced the severity of clinical signs induced by LPS, caused significant increase in PO2, SatO2 and IL-10 and significant decrease in PCO2, the total protein, TNF-α,IL-6, Ig M, albumin, WBCs, neutrophils and macrophages at different times of sampling both in compared with before transplantation(time 0) and in compared with the group of control. While in the control group, inflammation continued until the end of the study. SignificanceThese results showed that BM-MSCs are able to reduce inflammation and have an important role in reconstruction of the damaged lung.
Highlights
Acute respiratory distress syndrome (ARDS) is a clinical concept that is defined by acute hypoxemic failure, bilateral widespread capillary leakage and low lung compliance 1
Culture and characterizations of bone marrow (BM)-mesenchymal stem/stromal cells (MSCs) in sheep Cells that are isolated from the BM were spherical early
Results of clinical and laboratory after BM-MSCs transplantation in ARDS sheep MSCs: recovery of clinical symptoms After the cell therapy in the treatment group (ARDS+BM-MSCs), a decrease in HR observed since time 6 hours
Summary
Acute respiratory distress syndrome (ARDS) is a clinical concept that is defined by acute hypoxemic failure, bilateral widespread capillary leakage and low lung compliance 1. ARDS is caused by response to multiple predisposing factors, including pulmonary and extrapulmonary factors and infectious and noninfectious agents, of which sepsis is the most important and accounts for 75% of these [3,4]. The heterogeneity, complexity and variety of the ARDS mechanism combined with the failure of current therapies for ARDS and the mortality rate 36-44% 5, require novel therapeutic interventions in ARDS that can target different mechanisms of injury and facilitate the lung repair. MSCs apply their favorable effects by release of paracrine factors, antimicrobial factors and up-regulating phagocytosis 6
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