Abstract
Objective: The present study focused on the identification of particular extract which shows signification protection of DNA sugar against excessive oxidation. Methods: The different extracts (water, alcohol, alcohol: water, and hexane) of Agathi seeds (Sesbania grandiflora Linn) were evaluated using various antioxidant and other relevant assays like DNA sugar protection and antioxidant activities. Results: The alcohol: water (1:1) extract of S. grandiflora Linn seeds showed the highest antioxidant and free radical scavenging activity. It inhibited membrane lipid peroxidation by 55% at 50 μg/ml, scavenged approximately 69% of hydroxyl and 1,1-diphenyl-2-picrylhydrazayl radicals at 2–3 fold lower concentrations compared to the other extracts. In addition, the alcohol: water extract inhibited ferrous sulfate: ascorbate-induced sugar oxidation of DNA and also showed non-toxic nature against lymphocytes. Conclusion: These results establish the antioxidant potential of the extract, which could be used as natural antioxidant source.
Highlights
Day by day, researchers are in the search of new source of natural antioxidants
Agathi seeds were obtained from authentic source, Bangalore Karnataka state, India. 100 g of well dried Agathi seeds were grounded into a fine powder using a domestic electric grinder
Ghost suspension (200 μg) and linolenic acid (1.8 μmole) were subjected to peroxidation by Fenton reactants (10:100 μmole) in a final volume of 0.5 mL Tris-buffered saline (TBS 100 mM, pH 7.4, and 0.15 M NaCl) with increasing concentration of agathi seeds extract (10 to 50 μg); the contents were incubated for an hour and 1% TBA was added
Summary
The alcohol: water (1:1) extract of S. grandiflora Linn seeds showed the highest antioxidant and free radical scavenging activity. It inhibited membrane lipid peroxidation by 55% at 50 μg/ml, scavenged approximately 69% of hydroxyl and 1,1-diphenyl-2-picrylhydrazayl radicals at 2–3 fold lower concentrations compared to the other extracts. The alcohol: water extract inhibited ferrous sulfate: ascorbate-induced sugar oxidation of DNA and showed non-toxic nature against lymphocytes
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