Abstract
Objective To evaluate the effect of diosmin and hesperidin flavonoids in the prevention of amphotericin B nephrotoxicity, through an experimental model on rats. Method Adult, male Wistar rats were distributed into the following groups: saline; diosmin hesperidin (animals that received 50 mg/kg of diosmin hesperidin, drinking water, for ten days); amphotericin B (animals that received 15 mg/kg/day of amphotericin B through intraperitoneal treatment for five days); amphotericin B+diosmin hesperidin. Renal function, fractional excretion of sodium; potassium and magnesium and oxidative metabolites were evaluated. Results Treatment with amphotericin B reduced renal function, as shown by the clearance of creatinine, increased tubular function markers and fractional excretion of sodium, potassium, magnesium and oxidative metabolites. Pre-treatment with diosmin hesperidin ameliorated clearance of creatinine and reduced tubular and oxidative injury. Conclusion Administration of amphotericin B resulted in reduction of renal function with tubular injury, and diosmin hesperidin showing an antioxidant protective effect on the kidneys.
Highlights
In 2004, the World Health organization launched the World Alliance for Patient Safety, with the objective of raising awareness and increasing political engagement for improving patient safety during healthcare[1].In Brazil, the governmental agency for patient safety, ANVISA (Agência Nacional de Vigilância Sanitária – the National Agency for Sanitary Care), promotes activities aimed at improving patient safety and the quality of health services
The animals were distributed into the following groups: Saline: these animals received 3 ml/kg of 0.9% physiological solution through an intraperitoneal (IP) injection, once a day, www.ee.usp.br/reeusp for five days; diosmin hesperidin (DH, n=9): the animals received 50 mg/kg of diosmin/hesperidin in drinking water for ten days; amphotericin B (Amp-B, n=6): the animals received 15 mg/kg of amphotericin B through IP once a day for five days; amphotericin B and diosmin hesperidin (Amp-B+DH, n=12): the animals were pre-medicated with a diosmin/hesperidin solution in their drinking water over a ten day period, and from the sixth day of the experiment, they began to receive Amp-B through a single dose IP injection over a period of five days
The pre-conditioning with diosmin and hesperidin in animals treated with Amp-B showed a significant reduction in the creatinine serum levels, resulting in increased clearance of creatinine (p
Summary
In 2004, the World Health organization launched the World Alliance for Patient Safety, with the objective of raising awareness and increasing political engagement for improving patient safety during healthcare[1]. In Brazil, the governmental agency for patient safety, ANVISA (Agência Nacional de Vigilância Sanitária – the National Agency for Sanitary Care), promotes activities aimed at improving patient safety and the quality of health services. An adverse reaction to drugs is understood to mean any negative or unwanted effect that arises after the administration of a normal dosage of the medication as used in clinical practice. This situation inserts nephrotoxicity induced by antimicrobial, chemotherapeutic, analgesic and immunosuppressive agents. This list should be added by diagnostic agents such as iodine and gadolinium radiocontrasts[3]
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