Prevention of 4-nitroquinoline 1-oxide-induced rat tongue carcinogenesis by garlic

Abstract

The present study was undertaken to examine the inhibitory effect of garlic ( Allium sativum) on 4-nitroquinoline 1-oxide (4NQO)-induced tongue carcinogenesis in male rats, both in the initiation and post-initiation phases. Lipid peroxidation, reduced glutathione (GSH), glutathione peroxidase (GPx), and glutathione S-transferase (GST) were used to monitor the chemopreventive potential of garlic. Biochemical estimations were carried out on tumour and normal tongue tissues. Diminished lipid peroxidation in the tumour tissue was accompanied by a significant increase in the levels of GSH, GPx and GST. Administration of garlic (250 mg/kg, p.o., three times a week) effectively suppressed 4NQO-induced tongue carcinogenesis as revealed by the absence of carcinomas in the initiation phase and their reduced incidence in the post-initiation phase. The results of the present study suggest that garlic may exert its chemopreventive effects by modulating lipid peroxidation and enhancing the levels of GSH, GPx and GST.