Abstract
In this study, the preventative effects of Lactobacillus plantarum YS-3 (LP-YS3) on colitis were studied using an in vitro animal experiment. Oxazolone was administered to BALB/c mice to induce colitis, and the preventive effects of LP-YS3 were determined using serum- and tissue-related indexes. The mice treated with LP-YS3 showed a significant decrease (p < 0.05) in disease activity index for colitis by inhibiting colon shortening and increasing colon weight/length ratio. The application of LP-YS3 resulted in a significant reduction in myeloperoxidase, nitric oxide, and malondialdehyde activities and a decrease in glutathione activity (p < 0.05) in mouse colon tissues. LP-YS3 also significantly increased serum interleukin-2 and reduced interleukin-10 cytokines levels in mice (p < 0.05). Reverse transcription-polymerase chain reaction and western blot assays showed that LP-YS3 application resulted in a significant increase in neuronal nitric oxide synthase, endothelial nitric oxide synthase, c-Kit, and stem cell factor expressions and a decrease in inducible nitric oxide synthase, interleukin-8, and C-X-C motif chemokine receptor 2 expressions in mouse colonic tissues (p < 0.05). These findings indicate that LP-YS3 imparts preventative effects on oxazolone-induced colitis in mice.
Highlights
Ulcerative colitis (UC) is an inflammatory bowel disease that increases the risk of colon cancer as well as significantly alters the quality and lifestyle of people within the age range of 20–50 years [1]
3 Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Huancheng North 2 Road 109, Guilin 541004, Guangxi, People’s Republic of China synthase, c-Kit, and stem cell factor expressions and a decrease in inducible nitric oxide synthase, interleukin-8, and C-X-C motif chemokine receptor 2 expressions in mouse colonic tissues (p \ 0.05). These findings indicate that Lactobacillus plantarum YS-3 (LP-YS3) imparts preventative effects on oxazoloneinduced colitis in mice
The isolated lactic acid bacteria were identified as L. plantarum YS3 (LP-YS3) from naturally fermented yak yoghurt which was obtained from a herdsman family in Qinghai–Tibet Plateau in Yushu, China, by our research team
Summary
Ulcerative colitis (UC) is an inflammatory bowel disease that increases the risk of colon cancer as well as significantly alters the quality and lifestyle of people within the age range of 20–50 years [1]. Clinical treatment using UC drugs imparts side effects when administered long term. There is a need to identify a safe and effective drug for the treatment of UC [3]. Recent investigations have shown that intestinal flora is strongly associated with UC by participating in eliciting mucosal immune responses, which is a hallmark of inflammatory bowel disease. Supplemental application of probiotics is considered as an effective technique in relieving symptoms of UC by regulating intestinal flora [4, 5]
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