Prevent Effects of Lactobacillus Fermentum HY01 on Dextran Sulfate Sodium-Induced Colitis in Mice.

Xiaoyong Chen,Jian Li,Hongwei Wang,Zelin Yang,Xin Zhao,Huayi Suo

doi:10.3390/nu9060545

Abstract

The aim of this study is to assess the preventive effects of Lactobacillus fermentum HY01 (LF-HY01) to dextran sulfate sodium induced-colitis. We observed the ratio of colon weight to its length, colon pathological changes, and the concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6) in serum. We also took account of the protein levels of IκBα, NF-κB p65, iNOS, and COX-2, and we measured the best effects of different doses of Lactobacillus fermentum HY01 (low dose group was 109 CFU/kg·bw, high dose group was 1010 CFU/kg·bw) on dextran sulfate sodium-induced colitis mice. The results were remarkable, suggesting that Lactobacillus fermentum HY01 had significant preventive effects in dextran sulfate sodium induced-colitis; simultaneously, the high dose group showed the best results among other groups. It can effectively alleviate the shortened colon length, reduce the ratio of colon weight to its length, reduce edema, inflammatory cells infiltration, and colon mucosa injury, and play an important role in the down-regulation of concentrations of pro-inflammatory factors (IFN-γ, IL-12, TNFα, and IL-6). Above all, Lactobacillus fermentum HY01 shows promising prevention for IκBα degradation, inhibition of NF-κB p65 phosphorylation cascades, and decreases the protein levels of iNOS and COX-2 as well.

Highlights

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) [1] which is a non-specific chronic inflammation of the intestine including immune disorders, genetic predisposition, environmental factors, and certain lifestyle deprivations [2,3,4]
The electrophoresis result of 16S rDNA amplification product from Lactobacillus fermentum HY01 (LF-HY01) was satisfactory with clear band, correct place, and non-specific amplification (Figure 1c)
The specific results were that LF-HY01 had 100% homology with Lactobacillus fermentum, and LF-HY01 gathered together with Lactobacillus fermentum in the Lactobacillus branch (Figure 1d)

Summary

Introduction

Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) [1] which is a non-specific chronic inflammation of the intestine including immune disorders, genetic predisposition, environmental factors, and certain lifestyle deprivations [2,3,4]. Dozens of different mouse models of colitis have been developed in order to study potential mechanisms of IBD pathogenesis and evaluate therapeutic effects. Among various different kinds of chemicals used to make colitis models, dextran sulfate sodium (DSS)-induced colitis is a widely used. DSS molecular weight is very critical for the severity of colitis; lower molecular weight (5 kDa) may result in milder colitis, while higher molecular weight (500 kDa) does not cause any injury to the colon. A molecular weight of 36–50 kDa is used to induce colitis [6]

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