Prevención del daño renal tras isquemia aguda mediante la administración de PGE1. Estudio morfométrico

Abstract

Summary Introduction. Warn renal ischemia is an inevitable consequence of a number of common clinical situations and operative procedures. Ischemic renal damage is a serious and unsolved problem in the subsequent fate of the occlusion of the renal artery. Material and methods. Fourty eight Wistar rats were used in the study. Through a midline abdominal incision, a right nephrectomy was performed in all animals. The left kidney was gently dissected and left attached only by its pedicle. The left kidney was the selectively infused via the angiocatheter with 0.01 mg of PGE1 in 2 ml of 0.9 per cent saline in the half of the animal and the other half the infusion is made only with saline. At the end of the infusion, the left renal artery was occluded with a microvascular clamp (warn renal ischemia). Studies were performed in four group of animals. Group consisted of 6 rats subjected to warn renal ischemia for 15 minutes. Group consisted of 6 rats subjected to warn renal ischemia for 60 minutes. Group consisted of 6 rats subjected to renal ischemia for 15 minutes and reperfusion at 24 hours. Group consisted of 6 rats subjected to renal ischemia for 15 minutes and reperfusion at 7 days. Group consisted of 6 rats subjected to renal ischemia for 60 minutes and reperfusion at 24 hours. Group consisted of 6 rats subjected to renal ischemia for 60 minutes and revascularization at 7 days. After completion of surgery, the rats were observed for recovery from the anesthesia and placed in cages, provided with standard rat chow and water ad libitum in the group of renal revascularization. The kidneys at the end of study were removed immediately after the period of ischemia. Slices of kidneys from these animals were immediately fixed by immersion in formaldehyde. Thick section for light microscopy were stained and studied histologic and morphologically. All values are expressed as mean, standard error of the mean. Student's t test was used for paired data. Results. The acute tubular necrosis that develops as a direct result of renal ischemia without protection in the group after 30 minutes of ischemia. Conclusions. Pretreatment with PGE1 of the kidneys previous to warn renal ischemia fails to attenuate the ischemic renal injury. This study, the showed that PGE1 had a little protective effect on the rat kidneys subjected to ischemia and reperfusion injury.